The mixture is 10% by weight. The mentioned research shows that properly calibrated 10% ordnance gelatin is a reliable tissue simulant. Calibration of ballistic gelatin is verified by firing a .177 steel BB at 590 feet per second (fps), plus or minus 15 fps, into the gelatin resulting in 8.5 centimeters (cm), plus or minus 1 cm, penetration (2.95" – 3.74"). During FBI tests any gelatin blocks which fail the calibration test are discarded. Calibration results are not published due to the Pass/Fail nature of the test.

• Weigh out exactly 2 lbs. of gelatin powder and place in a 1 gallon zip lock bag.
• Add 4 pounds of filtered tap water, temperature range 45°C (80°F).
• Eliminate the air while sealing the bag quickly.
• Gently mix the contents. NOTE: The contents of the bag will become very stiff within 10 minutes.
• Refrigerate the bag if it will not be used within 2 hours. The shelf life of the bag in the refrigerator is 72 hours.
• Bring at least 15 pounds of filtered water to a boil.
• Use a clean 5 gallon bucket with a lid. Place the bucket, without the lid, on a scale note the weight or tare of the scale.
• Break up and place the contents of the bag in the clean 5 gallon bucket.
• Add 2.5 ml of de-foamer on top of the mix.
• Place the bucket on the scale and carefully add 14 pounds of boiling water into the bucket until you have exactly 20 pounds of the mixture.
• Remember to account for the weight of the bucket, if the scale has not been tared.
• Gently stir the mixture for 30 seconds Place the lid on tight.
• Gently stir for 30 seconds every 15 minutes for the first hour. Leave for 4 hours. Let stand for 2-4 hours.
• After cooling the mixture for 4 hours, slowly pour the mixture into mold and place in refrigerator.
• A household refrigerator will take approximately 72 hours for the block to set properly.

NOTE: Do not open refrigerator often. Doing so will bring the inside temperature down. A commercial refrigerator will cool the block faster. Allow curing for 36 hours (note: larger blocks require longer cure time. (e.g. an 80lb block requires 96 hours to cure.)